Steps of the third generation IVF
The third generation IVF technology is an assisted reproductive technology, which is used to help couples who cannot conceive in a natural way to achieve their reproductive aspirations. For a long time, this technology has made great progress and been widely used in the medical field. The operation steps of the third generation IVF will be described in detail below.

1. Take eggs
The first step of the third generation IVF is to take eggs. This step is usually carried out in the woman's ovary under the guidance of ultrasound. Doctors will use a slender needle to puncture the woman's ovary to collect eggs. This process is usually carried out in an operating room and requires anesthesia.
During the egg retrieval process, doctors will monitor the follicles in the ovary through ultrasound imaging, and use slender needles to puncture the ovary to extract eggs. These eggs will be saved for the next step.
In the process of egg retrieval, some complications may occur sometimes, such as the risk of ovarian perforation or infection. Therefore, this step must be carried out by experienced doctors.
2. Sperm extraction
After egg retrieval, male semen samples need to be obtained. The man can collect semen samples in a specific room of the hospital using manual methods or by masturbation. In order to improve the success rate of obtaining semen samples, it is usually recommended that there should be no sexual behavior or masturbation 2 to 7 days before semen extraction. This is to enable the man to provide higher quality and more quantity of sperm when collecting semen.
The collected semen samples will be sent to the laboratory for further processing to obtain the best fertilization rate.
3. Fertilization and embryo culture
In the laboratory, high-quality eggs and semen samples are placed in a petri dish to facilitate the fertilization process. This can be achieved by injecting sperm into eggs, or by culturing sperm and eggs together in a specific culture medium.
After fertilization, the egg will remain in the culture dish for 48 to 72 hours until it develops into an embryo. During this period, laboratory technicians will regularly check the development of embryos and select the most suitable embryos for transplantation.
4. Embryo transfer and pregnancy
Embryo transfer is the last step of the third generation IVF operation. Usually, when the embryo develops to the appropriate stage, the doctor will select the embryo most likely to be successfully implanted and developed for transfer.
Embryo transfer is usually performed in the operating room of a hospital. Doctors will use a slender tube to carefully place the embryo into a woman's uterus. The whole process is usually very short and does not require anesthesia.
After embryo transfer, the woman needs to conduct close monitoring to determine whether the pregnancy is successful. This usually includes regular measurements of progesterone levels and ultrasound examinations. If pregnancy is successful, the goal of three generations of IVF will be achieved.
Summary
Three generation IVF technology includes egg retrieval, sperm retrieval, fertilization and embryo culture, as well as embryo transfer and pregnancy. These steps need to be carried out under the guidance of experienced doctors and laboratory technicians. Although there may be certain risks in each step, through strict operation and monitoring, the third-generation IVF technology has become an effective way to help couples who cannot conceive naturally realize their reproductive dreams.