Operation of sperm extraction from test tube infants
IVF is an assisted reproductive technology, that is, the eggs and sperm are artificially combined, cultured to the embryonic stage, and then the embryos are implanted into the female uterus. Sperm retrieval is the first step for IVF. The following will introduce IVF sperm retrieval operation in detail from four aspects: donor selection, sperm retrieval method, storage and transportation, and related precautions.
1. Selection of sperm donors
The choice of donors is one of the important factors for the success of IVF. Generally speaking, the hospital will strictly screen the donors, including health status, genetic history, semen quality and so on. Common donor requirements include:
1) Aged between 20-40;
2) No infectious disease or major genetic disease history;
3) Semen quality was good, including sperm quantity, motility and morphology;
4) Good medical background, physical examination and mental assessment.
After these screening conditions, qualified donors can conduct further sperm extraction operations.
2. Sperm extraction method
Sperm extraction is a simple process carried out in the general surgical operating room, which is usually divided into two methods, including surgical sperm extraction and self hatred sperm extraction.
Surgical sperm extraction is one of the commonly used methods of sperm extraction, which is mainly applicable to men who cannot ejaculate normally, such as those who cannot ejaculate due to Melanie's syndrome, spinal cord injury and other reasons. Surgical semen extraction includes testicular puncture and seminal vesicle puncture. In the process of surgical sperm extraction, doctors will use local anesthesia or general anesthesia to remove sperm from the testicle or seminal vesicle by puncture.
Self loathing semen extraction refers to the self loathing operation conducted by men under natural conditions, and semen is collected into containers. Men are usually required to remove semen manually or sexually after abstinence for 3-5 days. This is a common non-surgical method of semen extraction, which can be carried out at home, convenient and fast.
3. Storage and transportation
After sperm extraction, it needs to be preserved and transported to ensure its quality and vitality. Generally speaking, sperm can be stored in liquid nitrogen at - 196 ℃. The container for storing sperm is usually a liquid nitrogen tank, which has good sealing and shock resistance.
Sperm transport needs to be kept at low temperature. Hospitals usually provide professional transportation services, using dry ice or liquid nitrogen for insulation. During transportation, minimize vibration and mechanical collision to avoid adverse effects on sperm quality.
4. Precautions
In the process of sperm extraction, the following matters should also be noted:
1) Abstinence period before semen extraction: the donor needs to follow the requirements of the hospital and abstain properly before semen extraction;
2) Follow the doctor's instructions: During the process of sperm extraction, you need to follow the doctor's guidance and operation requirements;
3) Avoid excessive operation: during sperm sperm extraction, excessive operation should be avoided to avoid unnecessary damage to the testes;
4) Pay attention to nutrition and living habits: sperm quality is closely related to living habits. Donors should maintain good living habits and pay attention to balanced nutrition.
summary
Sperm retrieval is the first step for IVF. Through detailed introduction of donor screening, sperm retrieval methods, preservation and transportation, and related precautions, we hope that people can have a clearer understanding and understanding of IVF sperm retrieval operations. Reasonable donor screening, appropriate semen extraction methods, scientific preservation and transportation, and prudent operation and precautions will guarantee the success of IVF.
