1. Development of IVF technology
With the progress of science and technology, IVF technology has been widely used in the past few decades, helping many infertile couples realize their reproductive dreams. IVF technology is to artificially conceive by combining sperm and egg in vitro, cultivating them into embryos, and then implanting them into the mother's body. This technology enables couples who are unable to conceive through natural pregnancy for a variety of reasons to have a new birth choice.

The development of IVF technology also makes it possible to choose gender. The sex of the embryo can be known by genetic analysis of the embryo in the process of IVF pre screening. In this way, couples can choose male or female embryo implantation according to their own preferences and needs.
However, it should be noted that the development of IVF technology has not changed the natural gender distribution of male and female germ cells. This means that IVF technology itself cannot determine the gender ratio, but provides the possibility of gender selection on the basis of the existing gender ratio.
2. Formation mechanism of male and female embryos
Understanding the formation mechanism of male and female embryos is important for understanding the changes in the ratio of male to female in vitro babies. In the process of human reproduction, the sex of the fertilized egg is determined by the sperm. Sperm is divided into female sperm with X chromosome and male sperm with Y chromosome. When the sperm with X chromosome combines with the egg, the embryo will be female; When the sperm with Y chromosome combines with the egg, the embryo will be male.
This mechanism of male and female embryo formation has not changed in IVF technology. IVF technology only selects gender in the process of artificial pregnancy, and does not change the sex determination mechanism of the embryo itself. Therefore, the proportion of men and women that existed before the popularization of IVF has not changed significantly in IVF.
3. Analysis of figures and data
In order to have a more objective understanding of the changes in the ratio of male to female test tube babies, we can analyze some published data. Take a famous hospital in China as an example. In the IVF project completed by the hospital in 2020, 100 couples successfully gave birth to children through IVF technology. Among them, the proportion of boys is 53% and that of girls is 47%. This shows that in the sample of the hospital, the proportion of men and women is not significantly skewed.
If we comprehensively analyze the data released worldwide, we will find that the ratio of male and female IVF babies in different regions and hospitals is often different, but the overall ratio of male and female IVF babies has not changed significantly. This is because the IVF technology itself does not affect the formation mechanism of male and female embryos, but provides the possibility of sex selection in the process of artificial pregnancy.
4. Summary
By analyzing the development of IVF technology, the formation mechanism of male and female embryos and the actual data, we can draw a conclusion that the ratio of male and female in the second generation of IVF does not change over time. The IVF technology itself does not change the gender distribution of male and female germ cells, but provides the possibility of gender selection in the process of artificial pregnancy. Therefore, the male female ratio of IVF is still affected by natural factors and cannot be manipulated technically.
When choosing IVF, couples should start from the health of their children and their own wishes, rather than excessively pursuing the dreams of a certain gender. IVF technology is a great scientific and technological innovation, which provides hope and choice for couples who cannot conceive through natural pregnancy. We should treat and apply this technology with a positive and rational attitude, so that more couples can realize their reproductive dreams.