Operation process of single sperm test tube baby
Single sperm test tube baby is an assisted reproductive technology, which can help couples who cannot conceive naturally realize their reproductive dreams. The following is a detailed introduction to the operation process of IVF from four aspects.
1. Male semen collection and sperm screening
The first step of IVF is to collect male semen samples. This process is usually carried out in an isolated and clean environment to ensure the purity and quality of sampling. The collected semen samples will be sent to the laboratory for sperm screening and preparation to improve the success rate of fertilization.
In the laboratory, sperm will be screened by density gradient centrifugation. This is a commonly used method, through gradient centrifugation of different densities, the sperm with strong vitality can better settle to the bottom of the gradient. The quality of the selected sperm is good, and further operations can be carried out.
Next, observe and count the sperm under the laboratory microscope to determine the number and vitality of the collected sperm. In general, it is necessary to ensure that the number of sperm collected is sufficient and has good vitality.
2. Women's egg collection
The second step in IVF with single sperm is to collect women's eggs. In this step, multiple eggs are usually collected at the same time to improve the success rate of fertilization and obtain more embryos.
Women usually receive ovulation induction therapy, which promotes the ovarian production of multiple follicles. After timely monitoring the size and development of egg bubbles, determine the best collection time. Using ultrasound guided follicle puncture technology, the eggs are taken out through abdominal puncture or vaginal puncture.
The collected eggs will be immediately sent to the laboratory and placed in a petri dish for subsequent fertilization.
3. ICSI
In single sperm test tube infants, the fertilization process is usually performed by ICSI technology. This is a method of injecting single sperm directly into the egg to improve the success rate of fertilization.
ICSI technology needs to be operated under a microscope. An experienced laboratory technician will use a tiny glass tube to absorb the selected sperm and inject it into the egg. This process requires a high degree of technical experience and precise operation to ensure the smooth injection of sperm into the egg.
After the injection, the fertilized eggs are cultured in a specific culture medium to promote their continued development. Generally, the incubation time is 2-5 days.
4. Embryo transfer and pregnancy observation
After the embryo has reached the appropriate stage, it will be transferred. Embryo transfer is usually carried out via vagina or abdomen, depending on the specific conditions of doctors and patients.
After embryo transfer, the patient needs to continue to observe the pregnancy. The blood HCG is usually measured two weeks after transplantation to determine whether the pregnancy is successful. If the pregnancy is successful, the patient needs further prenatal care and examination.
Through the above operation process, the single sperm test tube baby technology has brought new hope to couples who cannot conceive naturally. Each step has been accurately operated and carefully monitored to improve the success rate of fertilization and finally realize the birth of a healthy and lovely baby.
Summary
The operation process of single sperm test tube baby includes male semen collection and sperm screening, female egg collection, neural sperm injection (ICSI), embryo transfer and pregnancy observation. Each step is very critical and requires precise operation by experienced medical staff. Single sperm test tube baby technology has brought hope and opportunity for couples who cannot conceive naturally.
