Three generation IVF process steps
The third generation IVF technology, also known as embryonic genomics screening (PGT), is an assisted reproductive technology to avoid the transmission of some genetic diseases. It is a method that can simultaneously detect the chromosome number and gene mutation of embryos. The following details the process steps of the third generation IVF.
1. Antibody screening and endocrine examination
Before the third generation IVF program, women need to undergo antibody screening and endocrine tests. Antibody screening is used to determine whether women are attacked by certain antibodies that affect pregnancy. Endocrine examination is to know whether women have endocrine disorders, because endocrine abnormalities may affect ovarian function and the production of ovarian hormones.
Some common antibody screening includes alpha fetoprotein antibody, antinuclear antibody, phospholipid antibody, etc. Common items of endocrine examination include serum estradiol, luteinizing hormone, follicle stimulating hormone, luteinizing hormone, etc.
If the results of antibody screening and endocrine test are normal, women can go to the next step.
2. Egg collection
Egg collection is one of the key steps of the third generation IVF program. Before egg collection, women need to undergo an insulin resistance test to ensure that their physical condition is suitable for egg collection.
Egg collection is usually carried out on the 12th to 14th days of the menstrual cycle, which needs to be carried out in the operating room of the hospital. Doctors will use ultrasound guided slender needle or puncture needle to puncture the ovary to obtain eggs. The eggs collected will be immediately sent to the laboratory for processing.
3. Sperm collection and sperm treatment
At the same time as egg collection, men need to provide semen samples. Semen samples can be collected by masturbation or using special condoms.
The semen samples collected will be processed in the laboratory. The purpose of treatment is to extract healthy and high-quality sperm from semen to improve fertilization rate. Treatment includes centrifugation, removal of inactive sperm and other unwanted substances from semen, and cultivation of sperm to obtain optimal quantity and quality.
4. Fertilization and embryo transfer
Fertilization and embryo transfer are the last step of the third generation IVF program. In the laboratory, eggs and sperm will be cultured in a specific medium to promote fertilization. After that, the embryos will continue to be cultured in the culture dish for at least three to five days until they reach the optimal embryonic development level.
Doctors will choose the best embryos for transplantation. Embryo transfer is usually carried out on the third or fifth day of a woman's menstrual cycle, and can be completed through vaginal or laparoscopic surgery. After transplantation, women need to rest for a period of time to promote embryo implantation.
Summary
The process steps of the third generation IVF include antibody screening and endocrine examination, egg collection, sperm collection and processing, and fertilization and embryo transfer. This process requires reasonable time arrangement and strict compliance with the guidance and requirements of professional doctors. Through three generations of IVF technology, we can reduce the transmission risk of some genetic diseases and increase the birth rate of healthy babies.
