Sperm source of IVF
Test tube baby technology is a kind of assisted reproduction technology, which is mainly applicable to the situation that both husband and wife have infertility or infertility problems. One of the key steps is to collect sperm for in vitro fertilization. The following will elaborate on the process of obtaining IVF sperm from four aspects.
Semen collection
Semen collection is the first step to obtain sperm. Generally, men need to collect manually or by themselves in the private environment of the hospital. Manual collection is to stimulate the penis erection manually, ejaculate when reaching the climax, and collect semen in a disposable container. Self collection is a process that men complete by themselves in the special room provided by the hospital. The hospital will provide a comfortable and private environment for men to collect. These methods need strict sanitary measures to ensure the quality of sperm collected is good.
Sperm separation and treatment
The collected semen needs to be separated and treated to obtain high-quality sperm. First, semen samples will be placed in a centrifuge for centrifugation to remove inactive or weakly active sperm, semen fluid and impurities from the sperm. Next, the hospital will use different methods to separate sperm with strong motility, such as density gradient centrifugation, gravity sedimentation or magnetic dynamic separation. The isolated high-quality sperm will be stored in the appropriate culture medium to prepare for the subsequent in vitro fertilization process.
Sperm preservation
In order to ensure the survival and quality of sperm, the hospital will store the sperm under low temperature conditions. A common method is liquid nitrogen cryopreservation. The hospital mixed high-quality sperm with a special cryoprotectant, sealed it with a small tube, then quickly frozen it to extremely low temperature, and stored it in a liquid nitrogen tank. This method can effectively maintain the motility and survival rate of sperm, and is convenient to use in the future in vitro fertilization.
Sperm use and in vitro fertilization
When the couple is ready for the IVF procedure, the hospital will unfreeze the sperm from the frozen storage and conduct in vitro fertilization. The thawed sperm will be prepared and treated to restore its best condition. In general, the hospital will place the thawed sperm and eggs together in a culture dish for in vitro fertilization in an artificially controlled environment. This process usually requires close monitoring and regulation to ensure the success rate of fertilization.
In conclusion, IVF sperm is mainly collected from semen. Then, the semen collected was separated and treated, and finally stored at low temperature. When the husband and wife are ready for the IVF procedure, the hospital will use the thawed sperm for in vitro fertilization. This process requires strict steps and professional operations to ensure the quality of sperm and the success of fertilization.
